Serrano, M., Gomez-Lahoz, E., DePinho, R. A., Beach, D., Bar-Sagi, D. (January 1995) Inhibition of ras-induced proliferation and cellular transformation by p16INK4. Science, 267 (5195). pp. 249-52. ISSN 0036-8075 (Print)
Abstract
The cyclin-dependent kinase 4 (CDK4) regulates progression through the G1 phase of the cell cycle. The activity of CDK4 is controlled by the opposing effects of the D-type cyclin, an activating subunit, and p16INK4, an inhibitory subunit. Ectopic expression of p16INK4 blocked entry into S phase of the cell cycle induced by oncogenic Ha-Ras, and this block was relieved by coexpression of a catalytically inactive CDK4 mutant. Expression of p16INK4 suppressed cellular transformation of primary rat embryo fibroblasts by oncogenic Ha-Ras and Myc, but not by Ha-Ras and E1a. Together, these observations provide direct evidence that p16INK4 can inhibit cell growth.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenovirus E1A Proteins/genetics/physiology Animals Carrier Proteins/genetics/ physiology Cell Division Cell Transformation, Neoplastic Cells, Cultured Cyclin-Dependent Kinase 4 Cyclin-Dependent Kinase Inhibitor p16 Cyclin-Dependent Kinases Genes, Reporter Genes, Retinoblastoma Genes, myc Genes, ras Plasmids Protein-Serine-Threonine Kinases/antagonists & inhibitors/metabolism Proto-Oncogene Proteins Rats Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Retinoblastoma Protein/physiology S Phase Trans-Activation (Genetics) Transfection Tumor Cells, Cultured ras Proteins/genetics/ physiology |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell proliferation organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts organism description > animal > mammal > rodent > rat organism description > animal > mammal > rodent > rat |
| CSHL Authors: | |
| Communities: | CSHL labs > Beach lab |
| Depositing User: | Jessica Koos |
| Date: | 13 January 1995 |
| Date Deposited: | 31 Jul 2014 14:46 |
| Last Modified: | 31 Jul 2014 14:46 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30641 |
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