Raf1 interaction with Cdc25 phosphatase ties mitogenic signal transduction to cell cycle activation

Galaktionov, K., Jessus, C., Beach, D. (May 1995) Raf1 interaction with Cdc25 phosphatase ties mitogenic signal transduction to cell cycle activation. Genes Dev, 9 (9). pp. 1046-58. ISSN 0890-9369 (Print)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/7744247

DOI: 10.1101/gad.9.9.1046

Abstract

The Ras and Raf1 proto-oncogenes transduce extracellular signals that promote cell growth. Cdc25 phosphatases activate the cell division cycle by dephosphorylation of critical threonine and tyrosine residues within the cyclin-dependent kinases. We show here that Cdc25 phosphatase associates with raf1 in somatic mammalian cells and in meiotic frog oocytes. Furthermore, Cdc25 phosphatase can be activated in vitro in a Raf1-dependent manner. We suggest that activation of the cell cycle by the Ras/Raf1 pathways might be mediated in part by Cdc25.

Item Type:	Paper
Uncontrolled Keywords:	Amino Acid Sequence Animals Cell Cycle Cell Cycle Proteins/analysis/ metabolism Cell Extracts Cell Line Cell Membrane/chemistry Fibroblasts Hela Cells Humans Meiosis Molecular Sequence Data Oocytes Phosphoprotein Phosphatase/analysis/ metabolism Phosphorylation Protein-Serine-Threonine Kinases/analysis/ metabolism Proto-Oncogene Proteins/analysis/ metabolism Proto-Oncogene Proteins c-raf Recombinant Fusion Proteins/biosynthesis/metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Signal Transduction/ physiology Spodoptera Xenopus cdc25 Phosphatase
Subjects:	bioinformatics > genomics and proteomics > design > amino acid design organism description > animal > insect > Spodoptera organism description > animal organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line organism description > animal > mammal > primates > hominids > human organs, tissues, organelles, cell types and functions > organelles, types and functions > meiosis bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types organs, tissues, organelles, cell types and functions > tissues types and functions > signal transduction organism description > animal > Frog > xenopus
CSHL Authors:	Beach, David H. Galaktionov, Konstantin Jessus, Catherine
Communities:	CSHL labs > Beach lab
Depositing User:	Jessica Koos
Date:	1 May 1995
Date Deposited:	15 Aug 2014 19:37
Last Modified:	15 Aug 2014 19:37
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/30592

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