Activation of CLN1 and CLN2 G1 cyclin gene expression by BCK2

Di Como, C. J., Chang, H., Arndt, K. T. (April 1995) Activation of CLN1 and CLN2 G1 cyclin gene expression by BCK2. Mol Cell Biol, 15 (4). pp. 1835-46. ISSN 0270-7306 (Print)

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Abstract

The Saccharomyces cerevisiae CLN3 protein, a G1 cyclin, positively regulates the expression of CLN1 and CLN2, two additional G1 cyclins whose expression during late G1 is activated, in part, by the transcription factors SWI4 and SWI6. We isolated 12 complementation groups of mutants that require CLN3. The members of one of these complementation groups have mutations in the BCK2 gene. In a wild-type CLN3 genetic background, bck2 mutants have a normal growth rate but have a larger cell size, are more sensitive to alpha-factor, and have a modest defect in the accumulation of CLN1 and CLN2 RNA. In the absence of CLN3, bck2 mutations cause an extremely slow growth rate: the cells accumulate in late G1 with very low levels of CLN1 and CLN2 RNA. The slow growth rate and long G1 delay of bck2 cln3 mutants are cured by heterologous expression of CLN2. Moreover, overexpression of BCK2 induces very high levels of CLN1, CLN2, and HCS26 RNAs. The results suggest that BCK2 and CLN3 provide parallel activation pathways for the expression of CLN1 and CLN2 during late G1.

Item Type:	Paper
Uncontrolled Keywords:	Cyclins/ biosynthesis Fungal Proteins/biosynthesis/ genetics G1 Phase/ genetics Gene Expression Regulation, Fungal Genes, Fungal/genetics Genetic Complementation Test Mutation Phosphoprotein Phosphatase/metabolism Phosphoproteins/biosynthesis/ genetics Promoter Regions (Genetics)/genetics Protein Kinase C/genetics Protein-Serine-Threonine Kinases/genetics RNA, Messenger/biosynthesis Recombinant Proteins/biosynthesis Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/ genetics Saccharomyces cerevisiae Proteins Transcription, Genetic
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription organism description > yeast > Saccharomyces bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > Protein kinase C
CSHL Authors:	Arndt, Kim T Di Como, Charles
Communities:	CSHL labs
Depositing User:	Jessica Koos
Date:	April 1995
Date Deposited:	15 Aug 2014 19:48
Last Modified:	15 Aug 2014 19:48
PMCID:	PMC230409
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/30590

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