Davey, S., Beach, D.
(October 1995)
RACH2, a novel human gene that complements a fission yeast cell cycle checkpoint mutation.
Mol Biol Cell, 6 (10).
pp. 1411-21.
ISSN 1059-1524 (Print)
Abstract
We have identified a novel human gene by virtue of its ability to complement the rad1-1 checkpoint mutant of Schizosaccharomyces pombe. This gene, called RACH2, rescues the temperature-sensitive lethality of a rad1-1 wee1-50 double mutant of S. pombe. Expression of RACH2 in S. pombe rad1-1 strains partially restores UV resistance to the rad1-1 mutant strain. Expression of RACH2 in a rad1-1 cdc25-22 double mutant partially restores the dose-dependent delay in mitotic entry after irradiation that is lost in rad1-1 checkpoint-deficient mutants. Overexpression of RACH2 in human tissue culture cells induces apoptosis.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Amino Acid Sequence
Apoptosis
Cell Cycle
Cell Cycle Proteins/genetics
Cell Line
Cloning, Molecular
DNA, Antisense
DNA-Binding Proteins
Endonucleases/ genetics
Fibroblasts
Fungal Proteins/biosynthesis/chemistry/ genetics/ physiology
Genes/ genetics
Genetic Complementation Test
Humans
Keratinocytes
Membrane Transport Proteins
Molecular Sequence Data
Mutation
Phosphoprotein Phosphatase/genetics
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Schizosaccharomyces/ cytology/genetics/radiation effects
Schizosaccharomyces pombe Proteins
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Ultraviolet Rays
cdc25 Phosphatase |
| Subjects: |
bioinformatics > genomics and proteomics > design > amino acid design
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organism description > animal > mammal > primates > hominids > human
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Beach lab |
| Depositing User: |
Jessica Koos
|
| Date: |
October 1995 |
| Date Deposited: |
12 Aug 2014 13:58 |
| Last Modified: |
12 Aug 2014 13:58 |
| PMCID: |
PMC301296 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/30587 |
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