Bolwig, G. M., Del Vecchio, M., Hannon, G., Tully, T. (October 1995) Molecular cloning of linotte in Drosophila: a novel gene that functions in adults during associative learning. Neuron, 15 (4). pp. 829-42. ISSN 0896-6273 (Print)
Abstract
The linotte (lio) gene was identified in a screen for mutations that disrupted 3 hr memory after olfactory associative learning, without affecting the perception of odors or electroshock. The mutagenesis yielded a transposon-tagged gene disruption, which allowed rapid cloning of genomic DNA. The lio transcription unit was identified via rescue of the lio1 learning/memory defect by induced expression of a lio+ transgene in adults. The perception of odors or electroshock remained normal when the lio+ transgene was expressed in these lio1 flies. Learning/memory remained normal when the lio+ transgene was expressed in wild-type (lio+) flies. The lio gene produces only one transcript, the level of expression of which varies throughout development. Sequence analysis indicates that lio encodes a novel protein.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Animals Animals, Genetically Modified Blotting, Southern Chromosome Mapping Cloning, Molecular DNA Probes DNA Transposable Elements Drosophila/embryology/ genetics/physiology Drosophila Proteins Genes, Insect Heat Larva/metabolism Learning/ physiology Memory/physiology Molecular Sequence Data Proteins/chemistry/ genetics/physiology Pupa/metabolism Receptor Protein-Tyrosine Kinases Research Support, Non-U.S. Gov't Restriction Mapping Smell |
| Subjects: | organism description > animal > insect > Drosophila bioinformatics > genomics and proteomics > design > protein design organism description > animal > developmental stage > adult organism description > animal Investigative techniques and equipment > cloning Investigative techniques and equipment > assays > cloning Investigative techniques and equipment > probes > DNA probes organism description > animal behavior > learning bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > linotte gene organism description > animal behavior > memory |
| CSHL Authors: | |
| Communities: | CSHL labs > Tully lab |
| Depositing User: | Jessica Koos |
| Date: | October 1995 |
| Date Deposited: | 15 Aug 2014 16:28 |
| Last Modified: | 15 Aug 2014 16:28 |
| URI: | https://repository.cshl.edu/id/eprint/30570 |
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