Two waves of de novo methylation during mouse germ cell development

Molaro, A., Falciatori, I., Hodges, E., Aravin, A. A., Marran, K., Rafii, S., McCombie, W. R., Smith, A. D., Hannon, G. J. (July 2014) Two waves of de novo methylation during mouse germ cell development. Genes and Development, 28 (14). pp. 1544-9. ISSN 08909369 (ISSN)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/25030694

DOI: 10.1101/gad.244350.114

Abstract

During development, mammalian germ cells reprogram their epigenomes via a genome-wide erasure and de novo rewriting of DNA methylation marks. We know little of how methylation patterns are specifically determined. The piRNA pathway is thought to target the bulk of retrotransposon methylation. Here we show that most retrotransposon sequences are modified by default de novo methylation. However, potentially active retrotransposon copies evade this initial wave, likely mimicking features of protein-coding genes. These elements remain transcriptionally active and become targets of piRNA-mediated methylation. Thus, we posit that these two waves play essential roles in resetting germ cell epigenomes at each generation.

Item Type:	Paper
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation organism description > animal > developmental stage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell organism description > animal > mammal > rodent > mouse
CSHL Authors:	Falciatori, Ilaria Hannon, Gregory J. Hodges, Emily Marran, Krista McCombie, W. Richard Molaro, Antoine
Communities:	CSHL Cancer Center Program > Cancer Genetics CSHL labs > Hannon lab CSHL labs > McCombie lab CSHL Cancer Center Shared Resources > DNA Sequencing Service
Depositing User:	Matt Covey
Date:	15 July 2014
Date Deposited:	25 Jul 2014 14:49
Last Modified:	04 Nov 2015 20:30
PMCID:	PMC4102761
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/30554

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