Pappin, D. J. C., Rahman, D., Hansen, H. F., Bartlet-Jones, M., Jeffery, W., Bleasby, A. J. (1996) Chemistry, Mass Spectrometry and Peptide-Mass Databases: Evolution of Methods for the Rapid Identification and Mapping of Cellular Proteins. In: Mass Spectrometry in the Biological Sciences. Humana Press, pp. 135-150. ISBN 978-1-4612-6671-6
Abstract
Large-format 2D gel electrophoresis systems have been developed that are capable of resolving several thousand cellular proteins in a matter of days [1,2]. For a number of years, a combination of Edman microsequence analysis and identification of proteins by staining with specific antibodies has been used to systematically categorize proteins and establish cellular databases [3–5]. There are, however, significant problems associated with these approaches. Most resolved proteins are only present in the low- to upper-femtomole range, significantly below the level at which automated sequencers can reliably operate [6, 7]. The relatively slow speed of the Edman process (one or two samples per machine per day) also means that the sheer number of proteins is too great to permit large-scale characterization within any useful period of time. The use of monoclonal antibodies, whilst both rapid and extremely sensitive, requires the ready availability of a large pool of antibody probes.
| Item Type: | Book Section |
|---|---|
| Subjects: | Investigative techniques and equipment > spectroscopy > mass spectrometry |
| CSHL Authors: | |
| Communities: | CSHL labs > Pappin lab |
| Depositing User: | Matt Covey |
| Date: | 1996 |
| Date Deposited: | 22 May 2014 19:43 |
| Last Modified: | 24 Feb 2017 17:10 |
| URI: | https://repository.cshl.edu/id/eprint/30142 |
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