Schneider, B. L., Yang, Q. H., Futcher, A. B. (April 1996) Linkage of replication to start by the Cdk inhibitor Sic1. Science, 272 (5261). pp. 560-2. ISSN 0036-8075 (Print)
Abstract
In Saccharomyces cerevisiae, three G1 cyclins (Clns) are important for Start, the event committing cells to division. Sic1, an inhibitor of C1b-Cdc28 kinases, became phosphorylated at Start, and this phosphorylation depended on the activity of Clns. Sic1 was subsequently lost, which depended on the activity of Clns and the ubiquitin-conjugating enzyme Cdc34. Inactivation of Sic1 was the only nonredundant essential function of Clns, because a sic1 deletion rescued the inviability of the cln1 cln2 cln3 triple mutant. In sic1 mutants, DNA replication became uncoupled from budding. Thus, Sic1 may be a substrate of Cln-Cdc28 complexes, and phosphorylation and proteolysis of Sic1 may regulate commitment to replication at Start.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | CDC28 Protein Kinase, S cerevisiae/antagonists & inhibitors/metabolism Cell Cycle Cyclins/metabolism DNA Replication DNA, Fungal/biosynthesis Enzyme Inhibitors/ metabolism Fungal Proteins/ metabolism Ligases/metabolism Phosphorylation Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/cytology/ metabolism Saccharomyces cerevisiae Proteins Ubiquitin-Protein Ligase Complexes Ubiquitin-Protein Ligases |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication organism description > yeast > Saccharomyces organs, tissues, organelles, cell types and functions > organelles, types and functions > anaphase organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation |
| CSHL Authors: | |
| Communities: | CSHL labs > Futcher lab |
| Depositing User: | Kathleen Darby |
| Date: | 26 April 1996 |
| Date Deposited: | 13 May 2014 16:03 |
| Last Modified: | 13 May 2014 16:03 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30106 |
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