Okamoto, K., Kamibayashi, C., Serrano, M., Prives, C., Mumby, M. C., Beach, D.
(November 1996)
p53-dependent association between cyclin G and the B' subunit of protein phosphatase 2A.
Molecular and Cellular Biology, 16 (11).
pp. 6593-602.
ISSN 0270-7306
Abstract
We and others previously showed that cyclin G is a transcriptional target of the p53 tumor suppressor protein. However, cellular proteins which might form a complex with cyclin G have not yet been identified. To gain insight into the biological role of cyclin G, we used the yeast two-hybrid screen and isolated two mouse cDNAs encoding cyclin G-interacting proteins. Interestingly, both positive cDNAs encoded B' regulatory subunits of protein phosphatase 2A (PP2A). One clone encodes B'alpha, while the other clone codes for a new member of the B' family, B'beta. B'beta is 70% identical to other members of the B' family. B'alpha associated both in vitro and in vivo with cyclin G but not with the other mammalian cyclins. Furthermore, cyclin G formed a complex with B'alpha only after induction of p53 in p53 temperature-sensitive cell lines. These results indicate that cyclin G forms a specific complex with the B' subunit of PP2A and that complex formation is regulated by p53. Potential roles for the cyclin G-B' complex in p53-mediated pathways are discussed.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Alternative Splicing
Amino Acid Sequence
Animals
Baculoviridae
COS Cells
Cell Line
Cloning, Molecular
Cyclins/chemistry/ metabolism
Macromolecular Substances
Mammals
Mice
Molecular Sequence Data
Open Reading Frames
Protein-Tyrosine-Phosphatase/chemistry/ metabolism
Recombinant Proteins/chemistry/metabolism
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Saccharomyces cerevisiae/growth & development
Sequence Homology, Amino Acid
Spodoptera
Transfection
Tumor Suppressor Protein p53/ metabolism |
| Subjects: |
organism description > animal > insect > Spodoptera
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > p53
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > protein phosphatase
Investigative techniques and equipment > transfection
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > tumor suppressor |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Beach lab |
| Depositing User: |
Kathleen Darby
|
| Date: |
November 1996 |
| Date Deposited: |
13 May 2014 15:11 |
| Last Modified: |
13 May 2014 15:11 |
| PMCID: |
PMC231661 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/30098 |
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