Pappin, D. J. (1997) Peptide mass fingerprinting using MALDI-TOF mass spectrometry. Methods in molecular biology (Clifton, N.J.), 64. pp. 165-173. ISSN 10643745 (ISSN)
Abstract
Large-format 2D gel electrophoresis systems in routine operation are capable of resolving several thousand cellular proteins in 1 or 2 d (1,2). For the last decade, a combination of Edman microsequence analysis and identification of proteins by staining with specific antibodies has been used to systematically identify proteins and establish cellular databases (3–5). There are, however, significant problems associated with these approaches. Most proteins are only present in the low- to upper-femtomole range, which is significantly below the level at which automated sequencers can reliably operate (6,7). The relatively slow speed of the Edman process also means that the number of proteins is too great to permit large-scale characterization within any useful period of time. The use of monoclonal antibodies, while both rapid and sensitive, requires the ready availability of a large pool of specific antibody probes.
| Item Type: | Paper |
|---|---|
| Additional Information: | Cited By (since 1996): 46 Export Date: 24 January 2013 Source: Scopus PubMed ID: 9116819 Language of Original Document: English Correspondence Address: Pappin, D.J. Chemicals/CAS: glutamyl endopeptidase, EC 3.4.21.19; Peptide Fragments; Proteins; Serine Endopeptidases, EC 3.4.21.- |
| Uncontrolled Keywords: | glutamyl endopeptidase peptide fragment protein serine proteinase article chemistry electrophoresis esterification factual database instrumentation mass spectrometry methodology peptide mapping Databases, Factual Peptide Fragments Proteins Serine Endopeptidases Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| Subjects: | bioinformatics > genomics and proteomics > design > protein network design > peptide design Investigative techniques and equipment > spectroscopy > mass spectrometry |
| CSHL Authors: | |
| Communities: | CSHL labs > Pappin lab |
| Depositing User: | Kathleen Darby |
| Date: | 1997 |
| Date Deposited: | 08 May 2014 14:35 |
| Last Modified: | 08 May 2014 14:35 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29982 |
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