Henry, R. W., Mittal, V., Ma, B., Kobayashi, R., Hernandez, N. (September 1998) SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III. Genes and Development, 12 (17). pp. 2664-72. ISSN 08909369 (ISSN)
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Abstract
The basal transcription factor SNAPc binds to the PSE, a core element in the RNA polymerase II and III human snRNA promoters. SNAPc contains at least four subunits, but it has not been possible to assemble a fully defined recombinant SNAPc. Here we reconstitute SNAPc from five recombinant subunits, SNAP43, SNAP45, SNAP50, SNAP190, and a newly identified subunit, SNAP19. This recombinant complex binds specifically to the PSE and directs both RNA polymerase II and III snRNA gene transcription. Thus, the same core SNAPc nucleates the assembly of two classes of initiation complexes.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Base Sequence Hela Cells Humans Macromolecular Substances Models, Molecular Molecular Sequence Data Promoter Regions (Genetics) RNA Polymerase II/ metabolism RNA Polymerase III/ metabolism RNA, Small Nuclear/ genetics/metabolism Recombinant Proteins/biosynthesis/chemistry/metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Transcription Factors/biosynthesis/chemistry/genetics/ metabolism Transcription, Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > SNAP protein bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: | |
| Communities: | CSHL labs > Hernandez lab CSHL labs > Kobayashi lab CSHL labs > Mittal lab CSHL labs > Wigler lab |
| Depositing User: | Kathleen Darby |
| Date: | 1 September 1998 |
| Date Deposited: | 01 May 2014 18:04 |
| Last Modified: | 07 May 2014 13:14 |
| PMCID: | PMC317148 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29894 |
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