Mittal, V., Ma, B., Hernandez, N. (July 1999) SNAP(c): a core promoter factor with a built-in DNA-binding damper that is deactivated by the Oct-1 POU domain. Genes & Development, 13 (14). pp. 1807-21. ISSN 0890-9369
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Abstract
snRNA gene transcription is activated in part by recruitment of SNAP(c) to the core promoter through protein-protein contacts with the POU domain of the enhancer-binding factor Oct-1. We show that a mini-SNAP(c) consisting of a subset of SNAP(c) subunits is capable of directing both RNA polymerase II (Pol II) and Pol III snRNA gene transcription. Mini-SNAP(c) cannot be recruited by Oct-1, but binds as efficiently to the promoter as SNAP(c) together with Oct-1 and directs activated RNA Pol III transcription. Thus, SNAP(c) represses its own binding to DNA, and repression is relieved by interactions with the Oct-1 POU domain that promote cooperative binding. We have shown previously that TBP also represses its own binding, and in that case repression is relieved by cooperative interactions with SNAP(c). This may represent a general mechanism to ensure that core promoter-binding factors, which have strikingly slow off-rates, are recruited specifically to promoter sequences rather than to cryptic-binding sites in the genome.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | DNA-Binding Proteins/ antagonists & inhibitors/ metabolism Humans POU Domain Factors Promoter Regions (Genetics) RNA Polymerase II/metabolism RNA Polymerase III/metabolism RNA, Small Nuclear/genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Transcription Factors/ metabolism Transcription, Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > SNAP protein |
| CSHL Authors: | |
| Communities: | CSHL labs > Hernandez lab CSHL labs > Mittal lab CSHL labs > Wigler lab |
| Depositing User: | Kathleen Darby |
| Date: | 15 July 1999 |
| Date Deposited: | 28 Apr 2014 14:15 |
| Last Modified: | 28 Apr 2014 14:15 |
| PMCID: | PMC316888 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29831 |
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