Hoss, M., Robins, P., Naven, T. J., Pappin, D. J., Sgouros, J., Lindahl, T. (July 1999) A human DNA editing enzyme homologous to the Escherichia coli DnaQ/MutD protein. EMBO Journal, 18 (13). pp. 3868-75. ISSN 0261-4189 (Print)0261-4189 (Linking)
Abstract
Mammalian DNA polymerases alpha and beta lack 3' exonuclease activity and are unable to edit errors after DNA synthesis. However, editing exonucleases can be functions of separate polypeptides. We isolated a widely distributed DNA-specific 3' exonuclease from rabbit liver nuclei, sequenced tryptic peptides by mass spectrometry, and identified the corresponding human open reading frame. The protein expressed from the cloned human sequence exhibits 3' exonuclease activity. The human clone shares sequence homology with the editing function of the Escherichia coli DNA polymerase III holoenzyme, i.e., the DnaQ/MutD protein, and weakly with the editing 3' exonuclease domain of eukaryotic DNA polymerase epsilon. The gene maps to human chromosome 3p21.2-21.3. In a reconstituted human DNA repair system containing DNA polymerase beta and DNA ligase III-XRCC1, accurate rejoining of a 3' mismatched base residue at a single-strand break is dependent on addition of the exonuclease.
| Item Type: | Paper |
|---|---|
| Additional Information: | Hoss, M Robins, P Naven, T J Pappin, D J Sgouros, J Lindahl, T Research Support, Non-U.S. Gov't England The EMBO journal EMBO J. 1999 Jul 1;18(13):3868-75. |
| Uncontrolled Keywords: | Amino Acid Sequence Animals Base Pair Mismatch/genetics Catalysis Cell Nucleus/enzymology Chromosomes, Human, Pair 3/genetics Cloning, Molecular DNA Ligases/metabolism DNA Polymerase II/chemistry/genetics DNA Polymerase III DNA Polymerase beta/metabolism DNA Repair/genetics DNA, Single-Stranded/genetics/metabolism Escherichia coli/enzymology/ genetics Escherichia coli Proteins Exodeoxyribonuclease V Exodeoxyribonucleases/chemistry/ genetics/isolation & purification/ metabolism Humans Mass Spectrometry Molecular Sequence Data Molecular Weight RNA, Messenger/genetics/metabolism Rabbits Recombinant Fusion Proteins/biosynthesis/isolation & purification/metabolism Sequence Homology, Amino Acid |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > DNA polymerase bioinformatics > genomics and proteomics > analysis and processing > molecular interaction processing Investigative techniques and equipment > cloning Investigative techniques and equipment > assays > cloning organism description > bacteria > escherichia coli Investigative techniques and equipment > spectroscopy > mass spectrometry bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > polypeptides |
| CSHL Authors: | |
| Communities: | CSHL labs > Pappin lab |
| Depositing User: | Kathleen Darby |
| Date: | 1 July 1999 |
| Date Deposited: | 28 Apr 2014 13:19 |
| Last Modified: | 28 Apr 2014 13:19 |
| PMCID: | 1171463 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29827 |
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