Eckersley-Maslin, M. A., Thybert, D., Bergmann, J. H., Marioni, J. C., Flicek, P., Spector, D. L.
(February 2014)
Random Monoallelic Gene Expression Increases upon Embryonic Stem Cell Differentiation.
Developmental Cell, 28 (4).
pp. 351-65.
ISSN 1534-5807
Abstract
Random autosomal monoallelic gene expression refers to the transcription of a gene from one of two homologous alleles. We assessed the dynamics of monoallelic expression during development through an allele-specific RNA-sequencing screen in clonal populations of hybrid mouse embryonic stem cells (ESCs) and neural progenitor cells (NPCs). We identified 67 and 376 inheritable autosomal random monoallelically expressed genes in ESCs and NPCs, respectively, a 5.6-fold increase upon differentiation. Although DNA methylation and nuclear positioning did not distinguish the active and inactive alleles, specific histone modifications were differentially enriched between the two alleles. Interestingly, expression levels of 8% of the monoallelically expressed genes remained similar between monoallelic and biallelic clones. These results support a model in which random monoallelic expression occurs stochastically during differentiation and, for some genes, is compensated for by the cell to maintain the required transcriptional output of these genes.
| Item Type: |
Paper
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| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > differentiation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > histone
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells |
| CSHL Authors: |
|
| Communities: |
CSHL Cancer Center Program > Gene Regulation and Cell Proliferation
CSHL Cancer Center Shared Resources > Microscopy Service
CSHL labs > Spector lab
School of Biological Sciences > Publications
CSHL Cancer Center Shared Resources > Functional Genomics and Genetics Service |
| Highlight: |
Spector, David L. |
| Depositing User: |
Matt Covey
|
| Date: |
24 February 2014 |
| Date Deposited: |
07 Mar 2014 16:40 |
| Last Modified: |
16 Jul 2021 18:11 |
| PMCID: |
PMC3955261 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/29567 |
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