Navaratnam, D. S., Fernando, F. S., Priddle, J. D., Giles, K., Clegg, S. M., Pappin, D. J., Craig, I., Smith, A. D. (May 2000) Hydrophobic protein that copurifies with human brain acetylcholinesterase: amino acid sequence, genomic organization, and chromosomal localization. J Neurochem, 74 (5). pp. 2146-53. ISSN 0022-3042 (Print)0022-3042 (Linking)
Abstract
The mechanism of attachment of acetylcholinesterase (AChE) to neuronal membranes in interneuronal synapses is poorly understood. We have isolated, sequenced, and cloned a hydrophobic protein that copurifies with AChE from human caudate nucleus and that we propose forms a part of a complex of membrane proteins attached to this enzyme. It is a short protein of 136 amino acids and has a molecular mass of 18 kDa. The sequence contains stretches of both hydrophobic and hydrophilic amino acids and two cysteine residues. Analysis of the genomic sequence reveals that the coding region is divided among five short exons. Fluorescence in situ hybridization localizes the gene to chromosome 6p21.32-p21.2. Northern blot analysis shows that this gene is widely expressed in the brain with an expression pattern that parallels that of AChE.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Acetylcholinesterase/ isolation & purification Amino Acid Sequence/genetics Base Sequence/genetics Blotting, Northern Brain/enzymology/ metabolism Chromosome Mapping Databases as Topic Expressed Sequence Tags Genome Humans Molecular Sequence Data Nerve Tissue Proteins/genetics/ isolation & purification |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes |
| CSHL Authors: | |
| Communities: | CSHL labs > Pappin lab |
| Depositing User: | Matt Covey |
| Date: | May 2000 |
| Date Deposited: | 31 Jan 2014 14:51 |
| Last Modified: | 31 Jan 2014 14:51 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29400 |
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