Lendvai, B., Stern, E. A., Chen, B., Svoboda, K.
(April 2000)
Experience-dependent plasticity of dendritic spines in the developing rat barrel cortex in vivo.
Nature, 404 (6780).
pp. 876-881.
ISSN 0028-0836
Abstract
Do changes in neuronal structure underlie cortical plasticity(1,2)? Here we used time-lapse two-photon microscopy(3,4) of pyramidal neurons in layer 2/3 of developing rat barrel cortex(5) to image the structural dynamics of dendritic spines and filopodia. We found that these protrusions were highly motile: spines and filopodia appeared, disappeared or changed shape over tens of minutes. To test whether sensory experience drives this motility we trimmed whiskers one to three days before imaging. Sensory deprivation markedly (similar to 40%) reduced protrusive motility in deprived regions of the barrel cortex during a critical period around postnatal days (P)11-13, but had no effect in younger (P8-10) or older (P14-16) animals. Unexpectedly, whisker trimming did not change the density, length or shape of spines and filopodia. However, sensory deprivation during the critical period degraded the tuning of layer 2/3 receptive fields. Thus sensory experience drives structural plasticity in dendrites, which may underlie the reorganization of neural circuits.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
CORTICAL PLASTICITY
PYRAMIDAL NEURONS
VISUAL-CORTEX
FIELD CORTEX
SYNAPTOGENESIS
ORGANIZATION
FILOPODIA
SYNAPSES
DYNAMICS
CLOSURE |
| Subjects: |
organs, tissues, organelles, cell types and functions > tissues types and functions > barrel cortex
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
Investigative techniques and equipment > microscopy > flourescence microscopy > two-photon excitation microscopy
organs, tissues, organelles, cell types and functions > tissues types and functions > visual cortex |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Svoboda lab |
| Depositing User: |
Matt Covey
|
| Date: |
April 2000 |
| Date Deposited: |
30 Jan 2014 21:33 |
| Last Modified: |
30 Jan 2014 21:33 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/29387 |
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