Minshull, J., Sun, H., Tonks, N. K., Murray, A. W.
(November 1994)
A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts.
Cell, 79 (3).
pp. 475-486.
ISSN 0092-8674
Abstract
Like early Xenopus embryos, extracts made from Xenopus eggs lack the cell cycle checkpoint that keeps anaphase from occurring before spindle assembly is complete. At very high densities of sperm nuclei, however, microtubule depolymerization arrests the extracts in mitosis. The arrested extracts have high levels of maturation-promoting factor activity, fail to degrade cyclin B, and contain activated ERK2/mitogen-activated protein (MAP) kinase. The addition of the purified MAP kinase-specific phosphatase MKP-1 demonstrates that MAP kinase activity is required for both the establishment and maintenance of the mitotic arrest induced by spindle depolymerization. Increased calcium concentrations, which release unfertilized frog eggs from their natural arrest in metaphase of meiosis II, have no effect on the mitotic arrest.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
ACTIVATED PROTEIN-KINASE
CELL-CYCLE ARREST
WILD-TYPE P53
MATURATION-PROMOTING FACTOR
IMMEDIATE-EARLY GENE
M-PHASE
SACCHAROMYCES-CEREVISIAE
TYROSINE PHOSPHORYLATION
MICROTUBULE
DYNAMICS
CHROMOSOME MOVEMENT |
| Subjects: |
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > mitotic spindle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organism description > animal > Frog > xenopus |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Tonks lab |
| Depositing User: |
Matt Covey
|
| Date: |
November 1994 |
| Date Deposited: |
18 Dec 2013 15:30 |
| Last Modified: |
18 Dec 2013 15:30 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/29067 |
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