Lam, M. H. C., Michell, B. J., Fodero-Tavoletti, M. T., Kemp, B. E., Tonks, N. K., Tiganis, T. (October 2001) Cellular stress regulates the nucleocytoplasmic distribution of the protein-tyrosine phosphatase TCPTP. Journal of Biological Chemistry, 276 (40). pp. 37700-37707. ISSN 0021-9258
Abstract
Specific cellular stresses, including hyperosmotic stress, caused a dramatic but reversible cytoplasmic accumulation of the otherwise nuclear 45-kDa variant of the protein-tyrosine phosphatase TCPTP (TC45). In the cytoplasm, TC45 dephosphorylated the epidermal growth factor receptor and down-regulated the hyperosmotic stress-induced activation of the c-Jun N-terminal kinase. The hyperosmotic stress-induced nuclear exit of TC45 was not inhibited by leptomycin B, indicating that TC45 nuclear exit was independent of the exportin CRM-1. Moreover, hyperosmotic stress did not induce the cytoplasmic accumulation of a green fluorescent protein-TC45 fusion protein that was too large to diffuse across the nuclear pore. Our results indicate that TC45 nuclear exit may occur by passive diffusion and that cellular stress may induce the cytoplasmic accumulation of TC45 by inhibiting nuclear import. Neither p42(Erk2) nor the stress-activated c-Jun N-terminal kinase or p38 mediated the stress-induced redistribution of TC45. We found that only those stresses that stimulated the metabolic stress-sensing enzyme AMP-activated protein kinase (AMPK) induced the redistribution of TC45. In addition, specific pharmacological activation of the AMPK was sufficient to cause the accumulation of TC45 in the cytoplasm. Our studies indicate that specific stress-activated signaling pathways that involve the AMPK can alter the nucleocytoplasmic distribution of TC45 and thus regulate TC45 function in vivo.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | NUCLEAR EXPORT SIGNALS GROWTH-FACTOR RECEPTOR IMPORTIN-BETA KINASE ACTIVATION TRANSPORT CRM1 PHOSPHORYLATION SEQUENCE CELLS |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes diseases & disorders > pulmonary disease > oxidative stress bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > protein tyrosine phosphatase |
| CSHL Authors: | |
| Communities: | CSHL labs > Tonks lab |
| Depositing User: | Matt Covey |
| Date: | October 2001 |
| Date Deposited: | 18 Dec 2013 19:42 |
| Last Modified: | 18 Dec 2013 19:42 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/29018 |
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