Degtyareva, N. P., Greenwell, P., Hofmann, E. R., Hengartner, M. O., Zhang, L., Culotti, J. G., Petes, T. D. (February 2002) Caenorhabditis elegans DNA mismatch repair gene msh-2 is required for microsatellite stability and maintenance of genome integrity. Proceedings of the National Academy of Sciences of the United States of America, 99 (4). pp. 2158-2163. ISSN 0027-8424
Preview |
PDF
Hengartner_PNAS_2002.pdf - Published Version Download (161kB) | Preview |
Abstract
Mismatch repair genes are important in maintaining the fidelity of DNA replication. To determine the function of the Caenorhabditis elegans homologue of the MSH2 mismatch repair gene (msh-2), we isolated a strain of C. elegans with an insertion of the transposable element Tc1 within msh-2. Early-passage msh-2 mutants were similar to wild-type worms with regard to lifespan and meiotic chromosome segregation but had slightly reduced fertility. The mutant worms had reduced DNA damage-induced germ-line apoptosis after genotoxic stress. The msh-2 mutants also had elevated levels of microsatellite instability and increased rates of reversion of the dominant unc-58(e665) mutation. In addition, serially passaged cultures of msh-2 worms died out much more quickly than those of wild-type worms. These results demonstrate that msh-2 function in C. elegans is important in regulating both short- and long-term genomic stability.
| Item Type: | Paper |
|---|---|
| Subjects: | organism description > animal > C elegans bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes |
| CSHL Authors: | |
| Communities: | CSHL labs > Hengartner lab |
| Depositing User: | Matt Covey |
| Date: | February 2002 |
| Date Deposited: | 08 Jan 2014 21:56 |
| Last Modified: | 10 Sep 2019 19:05 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/28695 |
Actions (login required)
 |
Administrator's edit/view item |