Lu, X. W., Wontakal, S. N., Kavi, H., Kim, B. J., Guzzardo, P. M., Emelyanov, A. V., Xu, N., Hannon, G. J., Zavadil, J., Fyodorov, D. V., Skoultchi, A. I.
(April 2013)
Drosophila H1 Regulates the Genetic Activity of Heterochromatin by Recruitment of Su(var)3-9.
Science, 340 (6128).
pp. 78-81.
ISSN 0036-8075
Abstract
Eukaryotic genomes harbor transposable elements and other repetitive sequences that must be silenced. Small RNA interference pathways play a major role in their repression. Here, we reveal another mechanism for silencing these sequences in Drosophila. Depleting the linker histone H1 in vivo leads to strong activation of these elements. H1-mediated silencing occurs in combination with the heterochromatin-specific histone H3 lysine 9 methyltransferase Su(var)3-9. H1 physically interacts with Su(var) 3-9 and recruits it to chromatin in vitro, which promotes H3 methylation. We propose that H1 plays a key role in silencing by tethering Su(var) 3-9 to heterochromatin. The tethering function of H1 adds to its established role as a regulator of chromatin compaction and accessibility.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
linker histone h1
transposable elements
melanogaster
chromatin
genome
phosphorylation
germline
binding
repeats
rnas |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > histone
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation > histone methylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Hannon lab
School of Biological Sciences > Publications
CSHL Cancer Center Program > Cancer Genetics |
| Depositing User: |
Matt Covey
|
| Date: |
April 2013 |
| Date Deposited: |
23 May 2013 14:19 |
| Last Modified: |
02 Oct 2019 15:11 |
| PMCID: |
PMC3756538 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/28328 |
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