Schmitt, A., Zink, M., Petroianu, G., May, B., Braus, D. F., Henn, F. A.
(August 2003)
Decreased gene expression of glial and neuronal glutamate transporters after chronic antipsychotic treatment in rat brain.
Neuroscience Letters, 347 (2).
pp. 81-4.
ISSN 03043940 (ISSN)
Abstract
Post-mortem studies in schizophrenic patients revealed alterations in glutamate transporter gene expression. The question concerning a possible influence of chronic antipsychotic treatment on these gene expressions, however, remained open. In this study, 11 rats per group were treated with either haloperidol (1.5 mg/kg per day) or clozapine (45 mg/kg per day) over a period of 6 months in doses comparable to clinic application. Gene expression of glial glutamate transporter EAAT2 and the predominantly neuronal glutamate transporter EAAT3 were examined by in situ hybridization. Clozapine significantly downregulated EAAT2 in hippocampal CA1 and parietal cortex and EAAT3 in the cingulate cortex compared to the expression upon haloperidol treatment and controls. Additionally, clozapine decreased EAAT2 in temporal, cingulate and frontal cortex and downregulated EAAT3 in cingulate and infralimbic cortex, striatum and hippocampal CA1 and CA2 compared to controls. Haloperidol downregulated EAAT2 in cingulate and frontal cortex and reduced EAAT3 in nucleus accumbens, infralimbic cortex and hippocampal CA2. We hypothesize that glutamate transporter downregulation by neuroleptics increases glutamatergic action at the postsynaptic neuron and thereby may be related to beneficial antipsychotic effects and side effects.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Amino Acid Transport System X-AG/genetics/ metabolism
Animals
Antipsychotic Agents/administration & dosage/ pharmacology
Brain/ drug effects/metabolism
Clozapine/pharmacology
Down-Regulation
Excitatory Amino Acid Transporter 2/genetics/ metabolism
Excitatory Amino Acid Transporter 3
Glutamate Plasma Membrane Transport Proteins
Haloperidol/pharmacology
In Situ Hybridization
Male
Neuroglia/ drug effects/metabolism
Neurons/ drug effects/metabolism
Rats
Rats, Sprague-Dawley
Symporters/genetics/ metabolism
Time Factors |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
diseases & disorders > mental disorders > personality disorders
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > glia cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > glia cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > glia cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > tissues types and functions > transport |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Henn lab |
| Depositing User: |
Matt Covey
|
| Date: |
21 August 2003 |
| Date Deposited: |
11 Jun 2013 18:46 |
| Last Modified: |
11 Jun 2013 18:46 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/27959 |
Actions (login required)
 |
Administrator's edit/view item |
