Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the master clock control gene CCA1

Gutiérrez, R. A., Stokes, T. L., Thum, K., Xu, X., Obertello, M., Katari, M. S., Tanurdzic, M., Dean, A., Nero, D. C., McClung, C. R., Coruzzi, G. M. (March 2008) Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the master clock control gene CCA1. Proc Natl Acad Sci U S A, 105 (12). pp. 4939-4944. ISSN 0027-8424

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Abstract

Understanding how nutrients affect gene expression will help us to understand the mechanisms controlling plant growth and development as a function of nutrient availability. Nitrate has been shown to serve as a signal for the control of gene expression in Arabidopsis. There is also evidence, on a gene-by-gene basis, that downstream products of nitrogen (N) assimilation such as glutamate (Glu) or glutamine (Gln) might serve as signals of organic N status that in turn regulate gene expression. To identify genome-wide responses to such organic N signals, Arabidopsis seedlings were transiently treated with ammonium nitrate in the presence or absence of MSX, an inhibitor of glutamine synthetase, resulting in a block of Glu/Gln synthesis. Genes that responded to organic N were identified as those whose response to ammonium nitrate treatment was blocked in the presence of MSX. We showed that some genes previously identified to be regulated by nitrate are under the control of an organic N-metabolite. Using an integrated network model of molecular interactions, we uncovered a subnetwork regulated by organic N that included CCA1 and target genes involved in N-assimilation. We validated some of the predicted interactions and showed that regulation of the master clock control gene CCA1 by Glu or a Glu-derived metabolite in turn regulates the expression of key N-assimilatory genes. Phase response curve analysis shows that distinct N-metabolites can advance or delay the CCA1 phase. Regulation of CCA1 by organic N signals may represent a novel input mechanism for N-nutrients to affect plant circadian clock function.

Item Type:	Paper
Uncontrolled Keywords:	circadian gene networks glutamate metabolism
Subjects:	organism description > plant > Arabidopsis bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics organs, tissues, organelles, cell types and functions > tissues types and functions > biological clock bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function organs, tissues, organelles, cell types and functions organism description > plant organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:	Tanurdzic, Milos
Communities:	CSHL labs > Martienssen lab
Depositing User:	Matt Covey
Date:	March 2008
Date Deposited:	25 Feb 2013 14:47
Last Modified:	08 Nov 2017 21:45
PMCID:	PMC2290744
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27629

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