Schlabach, M. R., Luo, J., Solimini, N. L., Hu, G., Xu, Q. K., Li, M. Z., Zhao, Z. M., Smogorzewska, A., Sowa, M. E., Ang, X. L. L., Westbrook, T. F., Liang, A. C., Chang, K., Hackett, J. A., Harper, J. W., Hannon, G. J., Elledge, S. J. (February 2008) Cancer proliferation gene discovery through functional Genomics. Science, 319 (5863). pp. 620-624. ISSN 0036-8075
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Abstract
Retroviral short hairpin RNA (shRNA) - mediated genetic screens in mammalian cells are powerful tools for discovering loss- of- function phenotypes. We describe a highly parallel multiplex methodology for screening large pools of shRNAs using half- hairpin barcodes for microarray deconvolution. We carried out dropout screens for shRNAs that affect cell proliferation and viability in cancer cells and normal cells. We identified many shRNAs to be antiproliferative that target core cellular processes, such as the cell cycle and protein translation, in all cells examined. Moreover, we identified genes that are selectively required for proliferation and survival in different cell lines. Our platform enables rapid and cost- effective genome-wide screens to identify cancer proliferation and survival genes for target discovery. Such efforts are complementary to the Cancer Genome Atlas and provide an alternative functional view of cancer cells.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | COLORECTAL CANCERS HUMAN BREAST P53 PATHWAY SCREEN PHOSPHATASE CELLS MDM2 |
| Subjects: | diseases & disorders > cancer bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification diseases & disorders bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > shRNA |
| CSHL Authors: | |
| Communities: | CSHL labs > Hannon lab |
| Depositing User: | Matt Covey |
| Date: | February 2008 |
| Date Deposited: | 28 Feb 2013 16:18 |
| Last Modified: | 03 Oct 2019 13:36 |
| PMCID: | PMC2981870 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27497 |
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