Genetic resources for maize cell wall biology

Penning, B. W., Hunter, C. T., Tayengwa, R., Eveland, A. L., Dugard, C. K., Olek, A. T., Vermerris, W., Koch, K. E., McCarty, D. R., Davis, M. F., Thomas, S. R., McCann, M. C., Carpita, N. C. (December 2009) Genetic resources for maize cell wall biology. Plant Physiology, 151 (4). pp. 1703-1728.

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DOI: 10.1104/pp.109.136804


Grass species represent a major source of food, feed, and fiber crops and potential feedstocks for biofuel production. Most of the biomass is contributed by cell walls that are distinct in composition from all other flowering plants. Identifying cell wallrelated genes and their functions underpins a fundamental understanding of growth and development in these species. Toward this goal, we are building a knowledge base of the maize (Zea mays) genes involved in cell wall biology, their expression profiles, and the phenotypic consequences of mutation. Over 750 maize genes were annotated and assembled into gene families predicted to function in cell wall biogenesis. Comparative genomics of maize, rice (Oryza sativa), and Arabidopsis (Arabidopsis thaliana) sequences reveal differences in gene family structure between grass species and a reference eudicot species. Analysis of transcript profile data for cell wall genes in developing maize ovaries revealed that expression within families differed by up to 100-fold. When transcriptional analyses of developing ovaries before pollination from Arabidopsis, rice, and maize were contrasted, distinct sets of cell wall genes were expressed in grasses. These differences in gene family structure and expression between Arabidopsis and the grasses underscore the requirement for a grass-specific genetic model for functional analyses. A UniformMu population proved to be an important resource in both forward- and reverse-genetics approaches to identify hundreds of mutants in cell wall genes. A forward screen of field-grown lines by near-infrared spectroscopic screen of mature leaves yielded several dozen lines with heritable spectroscopic phenotypes. Pyrolysis-molecular beam mass spectrometry confirmed that several nir mutants had altered carbohydrate-lignin compositions. © 2009 American Society of Plant Biologists.

Item Type: Paper
Uncontrolled Keywords: maize biofuel COMPARATIVE GENOMICS transcription analyses pyrolysis-molecular beam mass spectrometry
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > plant
CSHL Authors:
Communities: CSHL labs > Jackson lab
CSHL Post Doctoral Fellows
Depositing User: Matt Covey
Date: December 2009
Date Deposited: 21 Feb 2013 14:40
Last Modified: 01 May 2013 21:27
PMCID: PMC2785990
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