Sequencing the Connectome

Zador, A. M., Dubnau, J., Oyibo, H. K., Zhan, H., Cao, G., Peikon, I. D. (October 2012) Sequencing the Connectome. PLoS Biology, 10 (10). ISSN 1544-9173

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URL: http://www.ncbi.nlm.nih.gov/pubmed/23109909

DOI: 10.1371/journal.pbio.1001411

Abstract

Connectivity determines the function of neural circuits. Historically, circuit mapping has usually been viewed as a problem of microscopy, but no current method can achieve high-throughput mapping of entire circuits with single neuron precision. Here we describe a novel approach to determining connectivity. We propose BOINC ("barcoding of individual neuronal connections"), a method for converting the problem of connectivity into a form that can be read out by high-throughput DNA sequencing. The appeal of using sequencing is that its scale--sequencing billions of nucleotides per day is now routine--is a natural match to the complexity of neural circuits. An inexpensive high-throughput technique for establishing circuit connectivity at single neuron resolution could transform neuroscience research.

Item Type:	Paper
Subjects:	Investigative techniques and equipment Investigative techniques and equipment > assays organs, tissues, organelles, cell types and functions > organs types and functions > brain organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions Investigative techniques and equipment > whole exome sequencing Investigative techniques and equipment > assays > whole exome sequencing
CSHL Authors:	Cao, Gang Dubnau, Josh T. Oyibo, Hassana K. Peikon, Ian D. Zador, Anthony M. Zhan, Huiqing
Communities:	CSHL Post Doctoral Fellows CSHL labs > Dubnau lab CSHL labs > Zador lab School of Biological Sciences > Publications
Highlight:	Dubnau, Josh T.
Depositing User:	Matt Covey
Date:	October 2012
Date Deposited:	17 Jan 2013 17:21
Last Modified:	22 Sep 2014 20:49
PMCID:	PMC3479097
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27091

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