Wu, Q., Shigaki, T., Han, J. S., Kim, C. K., Hirschi, K. D., Park, S. (December 2012) Ectopic expression of a maize calreticulin mitigates calcium deficiency-like disorders in sCAX1-expressing tobacco and tomato. Plant Molecular Biology, 80 (6). pp. 609-619.
Abstract
Deregulated expression of an Arabidopsis H⁺/Ca²⁺ antiporter (sCAX1) in agricultural crops increases total calcium (Ca²⁺) but may result in yield losses due to Ca²⁺ deficiency-like symptoms. Here we demonstrate that co-expression of a maize calreticulin (CRT, a Ca²⁺ binding protein located at endoplasmic reticulum) in sCAX1-expressing tobacco and tomato plants mitigated these adverse effects while maintaining enhanced Ca²⁺ content. Co-expression of CRT and sCAX1 could alleviate the hypersensitivity to ion imbalance in tobacco plants. Furthermore, blossom-end rot (BER) in tomato may be linked to changes in CAX activity and enhanced CRT expression mitigated BER in sCAX1 expressing lines. These findings suggest that co-expressing Ca²⁺ transporters and binding proteins at different intracellular compartments can alter the content and distribution of Ca²⁺ within the plant matrix.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics organism description > plant > maize organism description > plant bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic plants |
| CSHL Authors: | |
| Communities: | CSHL Post Doctoral Fellows CSHL labs > Jackson lab |
| Depositing User: | Matt Covey |
| Date: | December 2012 |
| Date Deposited: | 17 Jan 2013 19:52 |
| Last Modified: | 03 May 2013 13:26 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27081 |
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