Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells

Paddison, P. J., Caudy, A. A., Bernstein, E., Hannon, G. J., Conklin, D. S. (2002) Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes and Development, 16 (8). pp. 948-958. ISSN 08909369 (ISSN)

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Abstract

RNA interference (RNAi) was first recognized in Caenorhabditis elegans as a biological response to exogenous double-stranded RNA (dsRNA), which induces sequence-specific gene silencing. RNAi represents a conserved regulatory motif, which is present in a wide range of eukaryotic organisms. Recently, we and others have shown that endogenously encoded triggers of gene silencing act through elements of the RNAi machinery to regulate the expression of protein-coding genes. These small temporal RNAs (stRNAs) are transcribed as short hairpin precursors (∼70 nt), processed into active, 21-nt RNAs by Dicer, and recognize target mRNAs via base-pairing interactions. Here, we show that short hairpin RNAs (shRNAs) can be engineered to suppress the expression of desired genes in cultured Drosophila and mammalian cells. shRNAs can be synthesized exogenously or can be transcribed from RNA polymerase III promoters in vivo, thus permitting the construction of continuous cell lines or transgenic animals in which RNAi enforces stable and heritable gene silencing.

Item Type: Paper
Uncontrolled Keywords: Gene silencing miRNA RNAi shRNA siRNA DNA directed RNA polymerase III double stranded RNA messenger RNA RNA short hairpin RNA unclassified drug animal cell article Caenorhabditis elegans cell line DNA sequence Drosophila gene expression regulation genetic engineering human human cell mammal cell nonhuman priority journal RNA transcription transgenic animal 3T3 Cells Animals Conserved Sequence COS Cells Endoribonucleases Gene Expression Humans Mice Ribonuclease III RNA Polymerase III RNA Processing, Post-Transcriptional RNA, Messenger Substrate Specificity Transfection Animalia Caenorhabditis Eukaryota Invertebrata Mammalia
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene silencing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > animal > mammal
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > shRNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
School of Biological Sciences > Publications
Depositing User: Matt Covey
Date: 2002
Date Deposited: 09 Jan 2013 16:56
Last Modified: 19 Sep 2014 14:12
PMCID: PMC152352
Related URLs:
URI: https://repository.cshl.edu/id/eprint/26466

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