Paddison, P. J., Hannon, G. J. (2003) siRNAs and shRNAs: Skeleton keys to the human genome. Current Opinion in Molecular Therapeutics, 5 (3). pp. 217-224. ISSN 14648431 (ISSN)
Abstract
The use of RNA interference (RNAi) to evoke gene silencing in mammalian cell systems is rapidly becoming a standard laboratory technique. Recent experiments have also proven the utility of RNAi in animals. The next few years will bear witness to the first RNAi-based genome-wide screens in mammals, breaking the barrier to the use of mammalian cells as a genetic system. In this review, some of the key features of RNAi in mammalian systems are discussed.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Functional genomics Gene silencing Human disease Mouse models RNAi shRNA siRNA double stranded RNA messenger RNA nuclease ribonuclease III RNA RNA helicase gene expression regulation gene targeting genetic screening genome analysis genomics human human genome laboratory test mammal cell nonhuman review RNA analysis RNA structure RNA translation standard Animals Genome, Human Humans Nucleic Acid Conformation RNA Interference RNA, Double-Stranded RNA, Small Interfering Animalia Mammalia |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > shRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA |
| CSHL Authors: | |
| Communities: | CSHL labs > Hannon lab School of Biological Sciences > Publications |
| Depositing User: | Matt Covey |
| Date: | 2003 |
| Date Deposited: | 09 Jan 2013 16:51 |
| Last Modified: | 19 Sep 2014 14:44 |
| URI: | https://repository.cshl.edu/id/eprint/26457 |
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