Tam, O. H., Aravin, A. A., Stein, P., Girard, A., Murchison, E. P., Cheloufi, S., Hodges, E., Anger, M., Sachidanandam, R., Schultz, R. M., Hannon, G. J.
(2008)
Pseudogene-derived small interfering RNAs regulate gene expression in mouse oocytes.
Nature, 453 (7194).
pp. 534-538.
ISSN 00280836 (ISSN)
Abstract
Pseudogenes populate the mammalian genome as remnants of artefactual incorporation of coding messenger RNAs into transposon pathways. Here we show that a subset of pseudogenes generates endogenous small interfering RNAs (endo-siRNAs) in mouse oocytes. These endo-siRNAs are often processed from double-stranded RNAs formed by hybridization of spliced transcripts from protein-coding genes to antisense transcripts from homologous pseudogenes. An inverted repeat pseudogene can also generate abundant small RNAs directly. A second class of endo-siRNAs may enforce repression of mobile genetic elements, acting together with Piwi-interacting RNAs. Loss of Dicer, a protein integral to small RNA production, increases expression of endo-siRNA targets, demonstrating their regulatory activity. Our findings indicate a function for pseudogenes in regulating gene expression by means of the RNA interference pathway and may, in part, explain the evolutionary pressure to conserve argonaute-mediated catalysis in mammals. ©2008 Nature Publishing Group.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
dicer
small interfering RNA
catalysis
evolution
gene expression
hybridization
protein
RNA
rodent
animal cell
article
controlled study
gene control
gene silencing
nonhuman
oocyte
phenotype
priority journal
pseudogene
Animals
Computational Biology
DNA Transposable Elements
Female
Gene Expression Regulation, Developmental
Gene Library
Mice
Oocytes
Pseudogenes
Ribonuclease III
RNA Interference
RNA, Messenger
RNA, Small Interfering
Mammalia |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > animal > mammal
organism description > animal > mammal > rodent > mouse
organism description > animal > mammal > rodent
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Hannon lab
CSHL labs > Sachidanandam lab
School of Biological Sciences > Publications |
| Depositing User: |
Matt Covey
|
| Date: |
2008 |
| Date Deposited: |
09 Jan 2013 16:54 |
| Last Modified: |
22 Sep 2014 16:21 |
| PMCID: |
PMC2981145 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/26446 |
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