Ni, J. Q., Zhou, R., Czech, B., Liu, L. P., Holderbaum, L., Yang-Zhou, D., Shim, H. S., Tao, R., Handler, D., Karpowicz, P., Binari, R., Booker, M., Brennecke, J., Perkins, L. A., Hannon, G. J., Perrimon, N.
(2011)
A genome-scale shRNA resource for transgenic RNAi in Drosophila.
Nature Methods, 8 (5).
pp. 405-407.
ISSN 15487091 (ISSN)
Abstract
Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource. © 2011 Nature America, Inc. All rights reserved.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
microRNA
short hairpin RNA
article
Drosophila
gene expression
gene silencing
nonhuman
oocyte development
priority journal
RNA interference
Animals
Animals, Genetically Modified
Base Sequence
DNA Primers
Drosophila melanogaster
Female
Gene Knockdown Techniques
Genetic Techniques
Genetic Vectors
Genome, Insect
MicroRNAs
Oogenesis
RNA, Small Interfering |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > design > nucleic acid design
bioinformatics > genomics and proteomics > design > nucleic acid design > RNAI Design
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > shRNA |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Hannon lab
CSHL Cancer Center Shared Resources > Bioinformatics Service
CSHL Cancer Center Shared Resources > DNA Sequencing Service |
| Depositing User: |
Matt Covey
|
| Date: |
2011 |
| Date Deposited: |
09 Jan 2013 16:15 |
| Last Modified: |
03 Oct 2019 14:49 |
| PMCID: |
PMC3489273 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/26428 |
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