Kim, Il-Han, Chen, Yi-Chun M., Spector, David L., Eils, Roland, Rohr, Karl (2011) Nonrigid registration of 2-D and 3-D dynamic cell nuclei images for improved classification of subcellular particle motion. IEEE Trans Image Process., 20 (4). pp. 1011-1022. ISSN 1941-0042
Abstract
The observed motion of subcellular particles in fluorescence microscopy image sequences of live cells is generally a superposition of the motion and deformation of the cell and the motion of the particles. Decoupling the two types of movements to enable accurate classification of the particle motion requires the application of registration algorithms. We have developed an intensity-based approach for nonrigid registration of multichannel microscopy image sequences of cell nuclei. First, based on 3-D synthetic images we demonstrate that cell nucleus deformations change the observed motion types of particles and that our approach allows to recover the original motion. Second, we have successfully applied our approach to register 2-D and 3-D real microscopy image sequences. A quantitative experimental comparison with previous approaches for nonrigid registration of cell microscopy has also been performed.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Algorithms Animals Artificial Intelligence Cell Nucleus Cell Tracking Humans Image Enhancement Image Interpretation, Computer-Assisted Imaging, Three-Dimensional Microscopy Motion Pattern Recognition, Automated Reproducibility of Results Sensitivity and Specificity Subtraction Technique |
| Subjects: | Investigative techniques and equipment > microscopy > flourescence microscopy Investigative techniques and equipment > microscopy > immunoflourescence microscopy Investigative techniques and equipment > microscopy |
| CSHL Authors: | |
| Communities: | CSHL Cancer Center Shared Resources > DNA Sequencing Service CSHL Cancer Center Shared Resources > Microscopy Service CSHL labs > Spector lab CSHL Cancer Center Program > Gene Regulation and Cell Proliferation |
| Depositing User: | Matt Covey |
| Date: | 2011 |
| Date Deposited: | 10 Dec 2012 20:03 |
| Last Modified: | 13 Oct 2015 14:47 |
| PMCID: | PMC3282047 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26369 |
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