Spector, D. L. (1996) Nuclear organization and gene expression. Experimental Cell Research, 229 (2). pp. 189-197. ISSN 0014-4827
Abstract
In actively transcribing cells, factors involved in pre-mRNA splicing localize in a speckled pattern at the fluorescence microscopic level. The speckled pattern corresponds to interchromatin granule clusters and perichromatin fibrils at the electron microscopic level. Based upon [3H]uridine incorporation studies transcription is thought to occur at the perichromatin fibrils and not within interchromatin granule clusters. We have shown that upon inhibition of RNA polymerase II transcription or pre-mRNA splicing, splicing factors redistribute and preferentially localize to interchromatin granule clusters, which become larger and more uniform in shape. Introduction of exogenous DNA templates into the cell nucleus results in a recruitment of splicing factors to the new sites of transcription. These data suggest that splicing factors are localized at storage and/or reassembly sites (interchromatin granule clusters) and are recruited to active sites of transcription (perichromatin fibrils). Furthermore, these data demonstrate that the speckled localization of pre-mRNA splicing factors is a reflection of the transcriptional and pre-mRNA splicing activities of the cell.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Cell Nucleus Gene Expression Humans In Situ Hybridization, Fluorescence Introns Microscopy, Immunoelectron Models, Genetic Nuclear Proteins RNA Precursors RNA Splicing Transcription, Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA |
| CSHL Authors: | |
| Depositing User: | Matt Covey |
| Date: | 1996 |
| Date Deposited: | 10 Dec 2012 19:57 |
| Last Modified: | 29 Jan 2015 19:29 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26334 |
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