Comparative genomic analysis of tumors: detection of DNA losses and amplification

Lisitsyn, N. A., Lisitsina, N. M., Dalbagni, G., Barker, P., Sanchez, C. A., Gnarra, J., Linehan, W. M., Reid, B. J., Wigler, M. H. (January 1995) Comparative genomic analysis of tumors: detection of DNA losses and amplification. Proc Natl Acad Sci U S A, 92 (1). pp. 151-5. ISSN 0027-8424 (Print)

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DOI: 10.1073/pnas.92.1.151


We demonstrate the use of representational difference analysis for cloning probes that detect DNA loss and amplification in tumors. Using DNA isolated from human tumor cell lines to drive hybridization against matched normal DNA, we were able to identify six genomic regions that are homozygously deleted in cultured cancer cells. When this method was applied in the reverse way, using normal DNA to drive hybridization against tumor cell DNA, we readily isolated probes detecting amplification. Representational difference analysis was also performed on DNAs derived from tumor biopsies, and we thereby discovered a probe detecting very frequent homozygous loss in colon cancer cell lines and located on chromosome 3p.

Item Type: Paper
Uncontrolled Keywords: Animals Astrocytoma genetics Base Sequence Bladder Neoplasms genetics Breast Neoplasms genetics Carcinoma Renal Cell genetics Cell Line Chromosome Deletion Chromosomes Human Pair 3 Colonic Neoplasms genetics Colorectal Neoplasms genetics Comparative Study DNA/ genetics DNA Primers DNA, Neoplasm genetics Glioblastoma genetics Humans Hybrid Cells In Situ Hybridization Fluorescence Kidney Neoplasms genetics Melanoma genetics Molecular Sequence Data Neoplasms genetics Polymerase Chain Reaction methods Rodentia Tumor Cells Cultured
Subjects: diseases & disorders > cancer
Investigative techniques and equipment
diseases & disorders > neoplasms
Investigative techniques and equipment > assays
Investigative techniques and equipment > probes > DNA probes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene amplification
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 3 January 1995
Date Deposited: 12 Apr 2012 14:27
Last Modified: 09 Nov 2017 19:32
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