Cloning the differences between two complex genomes

Lisitsyn, N., Lisitsyn, N., Wigler, M. H. (February 1993) Cloning the differences between two complex genomes. Science, 259 (5097). pp. 946-51. ISSN 0036-8075 (Print)

DOI: 10.1126/science.8438152


The analysis of the differences between two complex genomes holds promise for the discovery of infectious agents and probes useful for genetic studies. A system was developed in which subtractive and kinetic enrichment was used to purify restriction endonuclease fragments present in one population of DNA fragments but not in another. Application of this method to DNA populations of reduced complexity ("representations") resulted in the isolation of probes to viral genomes present as single copies in human DNA, and probes that detect polymorphisms between two individuals. In principle, this system, called representational difference analysis (RDA), may also be used for isolating probes linked to sites of genomic rearrangements, whether occurring spontaneously and resulting in genetic disorders or cancer, or programmed during differentiation and development.

Item Type: Paper
Uncontrolled Keywords: Adenoviridae genetics Bacteriophage lambda genetics Base Sequence Cloning Molecular Comparative Study DNA chemistry DNA Probes DNA Viral Female Gene Deletion Genetic Diseases Inborn genetics Humans Male Molecular Sequence Data Neoplasms genetics Nucleic Acid Hybridization methods Polymerase Chain Reaction Polymorphism Genetic Sequence Homology Nucleic Acid
Subjects: bioinformatics > genomics and proteomics > annotation > sequence annotation
bioinformatics > genomics and proteomics > Mapping and Rendering > Sequence Rendering
Investigative techniques and equipment > probes > DNA probes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes > genome annotation
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 12 February 1993
Date Deposited: 12 Apr 2012 14:15
Last Modified: 18 Nov 2016 17:24
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