Kawamukai, M., Gerst, J., Field, J., Riggs, M., Rodgers, L., Wigler, M. H., Young, D. (February 1992) Genetic and biochemical analysis of the adenylyl cyclase-associated protein, cap, in Schizosaccharomyces pombe. Molecular Biology of the Cell, 3 (2). pp. 167-180. ISSN 1059-1524
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Abstract
We have identified, cloned, and studied a gene, cap, encoding a protein that is associated with adenylyl cyclase in the fission yeast Schizosaccharomyces pombe. This protein shares significant sequence homology with the adenylyl cyclase-associated CAP protein in the yeast Saccharomyces cerevisiae. CAP is a bifunctional protein; the N-terminal domain appears to be involved in cellular responsiveness to RAS, whereas loss of the C-terminal portion is associated with morphological and nutritional defects. S. pombe cap can suppress phenotypes associated with deletion of the C-terminal CAP domain in S. cerevisiae but does not suppress phenotypes associated with deletion of the N-terminal domain. Analysis of cap disruptants also mapped the function of cap to two domains. The functional loss of the C-terminal region of S. pombe cap results in abnormal cellular morphology, slow growth, and failure to grow at 37-degrees-C. Increases in mating and sporulation were observed when the entire gene was disrupted. Overproduction of both cap and adenylyl cyclase results in highly elongated large cells that are sterile and have measurably higher levels of adenylyl cyclase activity. Our results indicate that cap is required for the proper function of S. pombe adenylyl cyclase but that the C-terminal domain of cap has other functions that are shared with the C-terminal domain of S. cerevisiae CAP.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | AFFINITY CAMP PHOSPHODIESTERASE affinity cAMP phosphodiesterase SACCHAROMYCES-CEREVISIAE Saccharomyces cerevisiae RAS PROTEINS DNA-SEQUENCE DNA sequence YEAST CLONING DOMAINS KINASE yeast cloning domains kinase |
| Subjects: | bioinformatics > genomics and proteomics > annotation > gene expression profiling annotation organism description > yeast > Saccharomyces bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein structure rendering |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab |
| Depositing User: | CSHL Librarian |
| Date: | February 1992 |
| Date Deposited: | 12 Apr 2012 15:10 |
| Last Modified: | 08 Nov 2016 21:36 |
| PMCID: | PMC275516 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26209 |
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