McCaffrey, A. P., Meuse, L., Pham, T. T. T., Conklin, D. S., Hannon, G. J., Kay, M. A. (July 2002) Gene expression - RNA interference in adult mice. Nature, 418 (6893). pp. 38-39. ISSN 0028-0836
DOI: 10.1038/418038a
Abstract
RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene silencing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > shRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA |
| CSHL Authors: | |
| Communities: | CSHL labs > Hannon lab |
| Depositing User: | Editor Margaret Fantz |
| Date: | 4 July 2002 |
| Date Deposited: | 17 Apr 2012 17:48 |
| Last Modified: | 01 Dec 2016 17:48 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26126 |
Actions (login required)
 |
Administrator's edit/view item |