Liu, J. D., Rivas, F. V., Wohlschlegel, J., Yates, J. R., Parker, R., Hannon, G. J.
(December 2005)
A role for the P-body component GW182 in microRNA function.
Nature Cell Biology, 7 (12).
pp. 1261-1266.
ISSN 1465-7392
Abstract
In animals, the majority of microRNAs regulate gene expression through the RNA interference (RNAi) machinery without inducing small-interfering RNA (siRNA)-directed mRNA cleavage(1). Thus, the mechanisms by which microRNAs repress their targets have remained elusive. Recently, Argonaute proteins, which are key RNAi effector components, and their target mRNAs were shown to localize to cytoplasmic foci known as P-bodies or GW-bodies(2,3). Here, we show that the Argonaute proteins physically interact with a key P-/GW-body subunit, GW182. Silencing of GW182 delocalizes resident P-/GW-body proteins and impairs the silencing of microRNA reporters. Moreover, mutations that prevent Argonaute proteins from localizing in P-/GW-bodies prevent translational repression of mRNAs even when Argonaute is tethered to its target in a siRNA-independent fashion. Thus, our results support a functional link between cytoplasmic P-bodies and the ability of a microRNA to repress expression of a target mRNA.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
MESSENGER RNA DEGRADATION
messenger rna degradation
PROCESSING BODIES
processing bodies
PROTEIN SYNTHESIS
protein synthesis
GW
gw
BODIES
bodies
C ELEGANS
c elegans
C elegans
TRANSLATION
translation
ARGONAUTE 2
argonaute 2
COMPLEX
complex
MIRNAS
mirnas
miRNA's
miRNA
TARGET
target |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Hannon lab |
| Depositing User: |
Editor Margaret Fantz
|
| Date: |
December 2005 |
| Date Deposited: |
16 Apr 2012 18:49 |
| Last Modified: |
01 Mar 2013 20:17 |
| PMCID: |
PMC1804202 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/26116 |
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