Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans

Ketting, R. F., Fischer, S. E. J., Bernstein, E., Sijen, T., Hannon, G. J., Plasterk, R. H. A. (October 2001) Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans. Genes and Development, 15 (20). pp. 2654-2659. ISSN 0890-9369

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Abstract

Double-stranded RNAs can suppress expression of homologous genes through an evolutionarily conserved process named RNA interference (RNAi) or post-transcriptional gene silencing (PTGS). One mechanism underlying silencing is degradation of target mRNAs by an RNP complex, which contains similar to 22 nt of siRNAs as guides to substrate selection. A bidentate nuclease called Dicer has been implicated as the protein responsible for siRNA production. Here we characterize the Caenorhabditis elegans ortholog of Dicer (K12H4.8; dcr-1) in vivo and in vitro. dcr-1 mutants show a defect in RNAi. Furthermore, a combination of phenotypic abnormalities and RNA analysis suggests a role for dcr-1 in a regulatory pathway comprised of small temporal RNA (let-7) and its target (e.g., lin-41).

Item Type: Paper
Uncontrolled Keywords: RNAi rnai siRNA sirna PTGS ptgs Dicer development DOUBLE STRANDED RNA double stranded rna CAENORHABDITIS ELEGANS caenorhabditis elegans REGULATORY RNA regulatory rna GENETIC genetic INTERFERENCE interference MESSENGER RNA messenger rna SUPPRESSION suppression EXPRESSION expression HELICASE helicase PROTEIN protein PLANTS plants
Subjects: organism description > animal > C elegans
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > dicer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > dicer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > dsRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Editor Margaret Fantz
Date: 15 October 2001
Date Deposited: 17 Apr 2012 19:32
Last Modified: 30 Nov 2016 21:26
PMCID: PMC312808
Related URLs:
URI: https://repository.cshl.edu/id/eprint/26111

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