Evidence for a functional link between profilin and CAP in the yeast S. cerevisiae

Vojtek, A., Haarer, B., Field, J., Gerst, J., Pollard, T. D., Brown, S., Wigler, M. H. (August 1991) Evidence for a functional link between profilin and CAP in the yeast S. cerevisiae. Cell, 66 (3). pp. 497-505. ISSN 0092-8674

URL: https://www.ncbi.nlm.nih.gov/pubmed/1868547
DOI: 10.1016/0092-8674(81)90013-1


CAP is a component of the S. cerevisiae adenylyl cyclase complex. The N-terminal domain is required for cellular RAS responsiveness. Loss of the C-terminal domain is associated with morphological and nutritional defects. Here we report that cap- cells bud randomly and are defective in actin distribution. The morphological and nutritional defects associated with loss of the CAP C-terminal domain are suppressed by over-expression of PFY, the gene encoding profilin, an actin- and polyphosphoinositide-binding protein. The phenotype of cells lacking PFY resembles that of cells lacking the CAP C-terminal domain. Study of mutated yeast profilins and profilins from Acanthamoeba suggests that the ability of profilin to suppress cap- cells is dependent upon a property other than, or in addition to, its ability to bind actin. This property may be its ability to bind polyphosphoinositides. We propose that CAP and profilin provide a link between growth signals and remodeling of the cellular cytoskeleton.

Item Type: Paper
Uncontrolled Keywords: Actins physiology Adenylate Cyclase physiology Amino Acid Sequence Base Sequence Cell Division Cloning, Molecular Contractile Proteins Cytoskeleton physiology DNA Mutational Analysis DNA, Fungal genetics Fungal Proteins metabolism Genes, Structural Fungal Microfilament Proteins physiology Molecular Sequence Data Oligonucleotides chemistry Restriction Mapping Saccharomyces cerevisiae physiology
Subjects: organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 9 August 1991
Date Deposited: 06 Apr 2012 14:29
Last Modified: 17 Nov 2016 17:11
Related URLs:
URI: https://repository.cshl.edu/id/eprint/25988

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