Wigler, M. H., Weinstein, I. B. (1975) Preparative Method for Obtaining Enucleated Mammalian-Cells. Biochemical and Biophysical Research Communications, 63 (3). pp. 669-674. ISSN 0006-291X
Abstract
Mouse L cells can be enucleated in suspension by centrifugation in discontinuous Ficoll density gradients while in the presence of Cytochalasin B. Greater than 50% of the cytoplasts thus obtained attach to glass or plastic and undergo morphologic recovery within 2–4 hours of replating. Protein synthesis in cytoplasts undergoes a biphasic decay from an initial rate of approximately 50% of control nucleated cells. This method can yield up to 5 × 108 cytoplasts with consistently low levels of contamination by nucleated cells (less than 0.2%), and is well suited for obtaining quantitative amounts of cytoplasts or karyoplasts for physiologic or biochemical studies.
| Item Type: | Paper |
|---|---|
| Subjects: | Investigative techniques and equipment organs, tissues, organelles, cell types and functions > cell types and functions |
| CSHL Authors: | |
| Communities: | CSHL labs > Wigler lab |
| Depositing User: | CSHL Librarian |
| Date: | 1975 |
| Date Deposited: | 06 Apr 2012 17:49 |
| Last Modified: | 02 May 2016 19:12 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25955 |
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