Yu, I. J., Spector, D. L., Bae, Y.-S., Marshak, D. R. (September 1991) Immunocytochemical localization of casein kinase II during interphase and mitosis. J Cell Biol, 114 (6). pp. 1217-32. ISSN 0021-9525
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Abstract
We have developed specific antibodies to synthetic peptide antigens that react with the individual subunits of casein kinase II (CKII). Using these antibodies, we studied the localization of CKII in asynchronous HeLa cells by immunofluorescence and immunoelectron microscopy. Further studies were done on HeLa cells arrested at the G1/S transition by hydroxyurea treatment. Our results indicate that the CKII alpha and beta subunits are localized in the cytoplasm during interphase and are distributed throughout the cell during mitosis. Further electron microscopic investigation revealed that CKII alpha subunit is associated with spindle fibers during metaphase and anaphase. In contrast, the CKII alpha' subunit is localized in the nucleus during G1 and in the cytoplasm during S. Taken together, our results suggest that CKII may play significant roles in cell division control by shifting its localization between the cytoplasm and nucleus.
| Item Type: | Paper |
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| Additional Information: | |
| Uncontrolled Keywords: | Amino Acid Sequence Animals Antibodies diagnostic use Brain enzymology Casein Kinases Cattle Fluorescent Antibody Technique Hela Cells cytology enzymology ultrastructure Humans Hydroxyurea pharmacology Interphase physiology Liver enzymology Microscopy Immunoelectron Mitosis drug effects physiology Molecular Sequence Data Organelles enzymology ultrastructure Peptides chemical synthesis immunology Protein Kinases analysis immunology isolation & purification |
| Subjects: | Investigative techniques and equipment Investigative techniques and equipment > microscopy > electron microscopy Investigative techniques and equipment > microscopy > flourescence microscopy Investigative techniques and equipment > microscopy > immunoflourescence microscopy bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase Investigative techniques and equipment > microscopy |
| CSHL Authors: | |
| Communities: | CSHL labs > Spector lab |
| Depositing User: | Brian Soldo |
| Date: | September 1991 |
| Date Deposited: | 30 Mar 2012 22:43 |
| Last Modified: | 29 Jan 2015 20:45 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25778 |
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