Spector, D. L., Watt, R. A., Sullivan, N. F. (March 1987) The v- and c-myc oncogene proteins colocalize in situ with small nuclear ribonucleoprotein particles. Oncogene, 1 (1). pp. 5-12. ISSN 0950-9232
Abstract
The intranuclear distribution of the v-myc and c-myc oncogene proteins were studied by immunofluorescence and immunoelectron microscopy. The nuclear distribution pattern of these proteins is shown to be identical to the distribution of small nuclear ribonucleoprotein particles (snRNPs). Colocalization was observed in cells expressing either the v- or c-myc proteins or in cells microinjected with the recombinant human c-myc protein. Immunolocalization studies revealed the v-myc protein and snRNPs to be concentrated within a nuclear network which excludes the nucleolus, nuclear pore-lamina complex, and portions of the nucleoplasm which contain the bulk of DNA. These results identify a nuclear region enriched in the myc-oncogene protein and snRNPs and raise the possibility that these nuclear constituents may function in related processes.
| Item Type: | Paper |
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| Additional Information: | |
| Uncontrolled Keywords: | Animals Cell Compartmentation Cell Nucleus metabolism ultrastructure Coturnix Fluorescent Antibody Technique Humans Microscopy, Electron Oncogene Proteins, Viral metabolism Proto-Oncogene Proteins metabolism RNA Processing Post Transcriptional Rats Ribonucleoproteins metabolism Ribonucleoproteins Small Nuclear |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > Myc Investigative techniques and equipment > microscopy > immunoflourescence microscopy bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene |
| CSHL Authors: | |
| Communities: | CSHL labs > Spector lab |
| Depositing User: | Brian Soldo |
| Date: | March 1987 |
| Date Deposited: | 30 Mar 2012 22:41 |
| Last Modified: | 29 Jan 2015 20:49 |
| URI: | https://repository.cshl.edu/id/eprint/25761 |
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