Wang, Y., Mamiya, A., Chiang, A. S., Zhong, Y. (April 2008) Imaging of an early memory trace in the Drosophila mushroom body. J Neurosci, 28 (17). pp. 4368-76. ISSN 1529-2401 (Electronic)
Preview |
PDF (Paper)
Zhong J Neurosci 2008.pdf - Published Version Download (535kB) | Preview |
Abstract
Extensive molecular, genetic, and anatomical analyses have suggested that olfactory memory is stored in the mushroom body (MB), a higher-order olfactory center in the insect brain. The MB comprises three subtypes of neurons with axons that extend into different lobes. A recent functional imaging study has revealed a long-term memory trace manifested as an increase in the Ca(2+) activity in an axonal branch of a subtype of MB neurons. However, early memory traces in the MB remain elusive. We report here learning-induced changes in Ca(2+) activities during early memory formation in a different subtype of MB neurons. We used three independent in vivo and in vitro preparations, and all of them showed that Ca(2+) activities in the axonal branches of alpha'/beta' neurons in response to a conditioned olfactory stimulus became larger compared with one that was not conditioned. The changes were dependent on proper G-protein signaling in the MB. The importance of these changes in the Ca(2+) activity of alpha'/beta' neurons during early memory formation was further tested behaviorally by disrupting G-protein signaling in these neurons or blocking their synaptic outputs during the learning and memory process. Our results suggest that increased Ca(2+) activity in response to a conditioned olfactory stimulus may be a neural correlate of early memory in the MB.
| Item Type: | Paper |
|---|---|
| Additional Information: | |
| Uncontrolled Keywords: | learning and memory Drosophila imaging calcium olfactory fluorescence microscopy |
| Subjects: | organism description > animal > insect > Drosophila organism description > animal behavior > memory organs, tissues, organelles, cell types and functions > tissues types and functions > mushroom body |
| CSHL Authors: | |
| Communities: | CSHL labs > Zhong lab |
| Depositing User: | CSHL Librarian |
| Date: | 23 April 2008 |
| Date Deposited: | 29 Mar 2012 19:15 |
| Last Modified: | 06 Nov 2017 21:04 |
| PMCID: | PMC3413309 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25592 |
Actions (login required)
 |
Administrator's edit/view item |