Genetic manipulation of the odor-evoked distributed neural activity in the Drosophila mushroom body

Wang, Y. L., Wright, N. J. D., Guo, H. F., Xie, Z. P., Svoboda, K., Malinow, R., Smith, D. P., Zhong, Y. (January 2001) Genetic manipulation of the odor-evoked distributed neural activity in the Drosophila mushroom body. Neuron, 29 (1). pp. 267-276. ISSN 0896-6273



Odor-induced neural activity was recorded by Ca2+ imaging in the cell body region of the Drosophila mushroom body (MB), which is the second relay of the olfactory central nervous system. The signals recorded are mainly from the cell layers on the brain surface because of the limited penetration of Ca2+-sensitive dyes. The densely packed cell bodies and their accessibility allow visualization of odor-induced population neural activity. It is revealed that odors evoke diffused neural activities in the MB. Although the signals cannot be attributed to individual neurons, patterns of the population neural activity can be analyzed. The activity pattern, but not the amplitude, of an odor-induced population response is specific for the chemical identity of an odor and its concentration. The distribution pattern of neural activity can be altered specifically by genetic manipulation of an odor binding protein and this alteration is closely associated with a behavioral defect of odor preference. These results suggest that the spatial pattern of the distributed neural activity may contribute to coding of odor information at the second relay of the olfactory system.

Item Type: Paper
Uncontrolled Keywords: OLFACTORY RECEPTOR NEURONS olfactory receptor neurons VOLTAGE-SENSITIVE DYE voltage sensitive dye ANTENNAL LOBE antennal lobe BULB bulb MELANOGASTER melanogaster RAT rat Drosophila COMBINATORIAL combinatorial STIMULATION stimulation RESPONSES responses BODIES bodies
Subjects: organism description > animal > insect > Drosophila
organs, tissues, organelles, cell types and functions > tissues types and functions > mushroom body
organism description > animal behavior > odor recognition
organism description > animal behavior > olfactory
CSHL Authors:
Communities: CSHL labs > Malinow lab
CSHL labs > Zhong lab
Depositing User: CSHL Librarian
Date: January 2001
Date Deposited: 29 Mar 2012 21:06
Last Modified: 29 Mar 2012 21:06

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving