Lucito, R., Nakimura, M., West, J. A., Han, Y., Chin, K., Jensen, K. R., McCombie, R. W., Gray, J. W., Wigler, M. H. (April 1998) Genetic analysis using genomic representations. Proceedings of the National Academy of Sciences of the United States of America, 95 (8). pp. 4487-92. ISSN 0027-8424
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Abstract
Analysis of the genetic changes in human tumors is often problematical because of the presence of normal stroma and the limited availability of pure tumor DNA. However, large amounts of highly reproducible "representations" of tumor and normal genomes can be made by PCR from nanogram amounts of restriction endonuclease cleaved DNA that has been ligated to oligonucleotide adaptors. We show here that representations are useful for many types of genetic analyses, including measuring relative gene copy number, loss of heterozygosity, and comparative genomic hybridization. Representations may be prepared even from sorted nuclei from fixed and archived tumor biopsies.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Blotting Southern Chromosome Deletion Chromosome Mapping DNA genetics DNA Neoplasm genetics Female Genes Tumor Suppressor Genes erbB-2 Genome Human Heterozygote Detection Humans Neoplasms genetics pathology Placenta Polymerase Chain Reaction methods Pregnancy Restriction Mapping |
| Subjects: | diseases & disorders > cancer Investigative techniques and equipment > cloning > PCR Investigative techniques and equipment > assays > cloning > PCR Investigative techniques and equipment > assays |
| CSHL Authors: | |
| Communities: | CSHL labs > Lucito lab CSHL labs > McCombie lab CSHL labs > Wigler lab |
| Depositing User: | Brian Soldo |
| Date: | 14 April 1998 |
| Date Deposited: | 29 Mar 2012 15:11 |
| Last Modified: | 11 Sep 2019 15:33 |
| PMCID: | PMC22516 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25556 |
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