Identification of alterations in DNA copy number in host stromal cells during tumor progression

Pelham, R. J., Rodgers, L., Hall, I. M., Lucito, R., Nguyen, K. C. Q., Navin, N. E., Hicks, J. B., Mu, D., Powers, S., Wigler, M. H., Botstein, D. (December 2006) Identification of alterations in DNA copy number in host stromal cells during tumor progression. Proc Natl Acad Sci U S A, 103 (52). pp. 19848-19853. ISSN 0027-8424

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URL: http://www.ncbi.nlm.nih.gov/pubmed/17167050

DOI: 10.1073/pnas.0609635104

Abstract

The interactions between cancer cells and the surrounding host stromal tissue play a critical role in tumor progression and metastasis, but the molecular nature of this relationship remains largely uncharacterized. Furthermore, although genetic changes of neoplastic cells in tumors contribute significantly to tumor progression, it is not known whether similar changes occur in the adjacent host stromal microenvironment and whether they contribute to or inhibit tumorigenesis. To address this question in an unbiased and genome-wide manner, we applied high-resolution DNA copy number analysis to murine stromal DNA isolated from human xenograft tumors that were formed in immunodeficient mice. We show that numerous amplifications and deletions are found within the host stromal microenvironment, suggesting that alterations in host DNA copy number can occur and may play a significant role in modifying tumor–stromal interactions.

Item Type:	Paper
Uncontrolled Keywords:	cancer stroma microarray
Subjects:	diseases & disorders > cancer diseases & disorders > neoplasms bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > copy number variants
CSHL Authors:	Hall, Ira M. Hicks, James B. Lucito, Robert Mu, David Navin, Nicholas E. Nguyen, Ken C. Q. Powers, Scott Rodgers, Linda Wigler, Michael H.
Communities:	CSHL labs > Hicks lab CSHL labs > Lucito lab CSHL labs > Powers lab CSHL labs > Wigler lab School of Biological Sciences > Publications
Depositing User:	Brian Soldo
Date:	December 2006
Date Deposited:	28 Mar 2012 19:17
Last Modified:	09 Nov 2017 16:04
PMCID:	PMC1698871
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/25543

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