Blake, P. R., Day, M. W., Hsu, B. T., Joshua-Tor, L., Park, J. B., Hare, D. R., Adams, M. W., Rees, D. C., Summers, M. F. (November 1992) Comparison of the X-ray structure of native rubredoxin from Pyrococcus furiosus with the NMR structure of the zinc-substituted protein. Protein Sci, 1 (11). pp. 1522-5. ISSN 0961-8368 (Print)0961-8368 (Linking)
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Abstract
The three-dimensional X-ray structures of the oxidized and reduced forms of rubredoxin from Pyrococcus furiosus, determined at -161 degrees C, and the NMR structure of the zinc-substituted protein, determined in solution at 45 degrees C, are compared. The NMR and X-ray structures, which were determined independently, are very similar and lead to similar conclusions regarding the interactions that confer hyperthermostability.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Archaea metabolism Magnetic Resonance Spectroscopy methods Models Molecular Protein Structure Secondary Rubredoxins chemistry metabolism X-Ray Diffraction methods Zinc metabolism |
| Subjects: | Investigative techniques and equipment > X-Ray Diffraction Investigative techniques and equipment > x ray crystallography |
| CSHL Authors: | |
| Communities: | CSHL labs > Joshua-Tor lab |
| Depositing User: | CSHL Librarian |
| Date: | November 1992 |
| Date Deposited: | 20 Mar 2012 19:14 |
| Last Modified: | 29 Aug 2017 20:20 |
| PMCID: | PMC2142123 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25507 |
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