Van Aalten, D. M. F., Crielaard, W., Hellingwerf, K. J., Joshua-Tor, L. (2002) Structure of the photoactive yellow protein reconstituted with caffeic acid at 1.16 Å resolution. Acta Crystallographica Section D: Biological Crystallography, 58 (4). pp. 585-590. ISSN 09074449 (ISSN) (Public Dataset)
Abstract
A structural study is described of the photoactive yellow protein (PYP) reconstituted with the chromophore derivative 3,4-dihydroxycinnamic acid. The crystal structure of PYP reconstituted with this chromophore at 1.16 Å resolution is reported in space group P65. This is the first high-resolution structure of a photoreceptor containing a modified chromophore. The introduction of an extra hydroxyl group in the native chromophore (i.e. p-coumaric acid) appears to perturb the structure of the hybrid yellow protein only slightly. The chromophore is bound by the protein in two different conformations, separated by a rotation of 180° of the catechol ring. In combination with available spectroscopic data, it is concluded that the caffeic acid chromophore binds to the protein in a strained conformation, which leads to a faster ejection from the chromophore-binding pocket upon pB formation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | bacterial protein caffeic acid caffeic acid derivative photoactive yellow protein, Bacteria visual proteins and pigments article chemical structure chemistry X ray crystallography Bacterial Proteins Caffeic Acids Crystallography X-Ray Models, Molecular Photoreceptors, Microbial |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein structure rendering Investigative techniques and equipment > x ray crystallography |
| CSHL Authors: | |
| Communities: | CSHL labs > Joshua-Tor lab |
| Depositing User: | CSHL Librarian |
| Date: | 2002 |
| Date Deposited: | 21 Mar 2012 19:47 |
| Last Modified: | 05 Sep 2017 19:15 |
| Related URLs: | |
| Dataset ID: | |
| URI: | https://repository.cshl.edu/id/eprint/25488 |
Actions (login required)
 |
Administrator's edit/view item |