High-affinity binding of Chp1 chromodomain to K9 methylated histone H3 is required to establish centromeric heterochromatin

Schalch, T., Job, G., Noffsinger, V. J., Shanker, S., Kuscu, C., Joshua-Tor, L., Partridge, J. F. (April 2009) High-affinity binding of Chp1 chromodomain to K9 methylated histone H3 is required to establish centromeric heterochromatin. Mol Cell, 34 (1). pp. 36-46. ISSN 1097-4164 (Electronic) (Public Dataset)

Abstract

In fission yeast, assembly of centromeric heterochromatin requires the RITS complex, which consists of Ago1, Tas3, Chp1, and siRNAs derived from centromeric repeats. Recruitment of RITS to centromeres has been proposed to depend on siRNA-dependent targeting of Ago1 to centromeric sequences. Previously, we demonstrated that methylated lysine 9 of histone H3 (H3K9me) acts upstream of siRNAs during heterochromatin establishment. Our crystal structure of Chp1's chromodomain in complex with a trimethylated lysine 9 H3 peptide reveals extensive sites of contact that contribute to Chp1's high-affinity binding. We found that this high-affinity binding is critical for the efficient establishment of centromeric heterochromatin, but preassembled heterochromatin can be maintained when Chp1's affinity for H3K9me is greatly reduced.

Item Type:	Paper
Additional Information:
Uncontrolled Keywords:	DNA Proteins
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome > centromere bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome > centromere bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics > heterochromatin bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA Investigative techniques and equipment > x ray crystallography organism description > yeast
CSHL Authors:	Joshua-Tor, Leemor
Communities:	CSHL labs > Joshua-Tor lab
Depositing User:	CSHL Librarian
Date:	10 April 2009
Date Deposited:	22 Mar 2012 14:56
Last Modified:	05 Sep 2017 16:21
PMCID:	PMC2705653
Related URLs:	Publisher
Dataset ID:	PDB: 3G7L
URI:	https://repository.cshl.edu/id/eprint/25467

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