Tripic, T., Deng, W., Cheng, Y., Zhang, Y., Vakoc, C. R., Gregory, G. D., Hardison, R. C., Blobel, G. A.
(2009)
SCL and associated proteins distinguish active from repressive GATA transcription factor complexes.
Blood, 113 (10).
pp. 2191-2201.
ISSN 00064971 (ISSN)
Abstract
GATA-1 controls hematopoietic development by activating and repressing gene transcription, yet the in vivo mechanisms that specify these opposite activities are unknown. By examining the composition of GATA-1-associated protein complexes in a conditional erythroid rescue system as well as through the use of tiling arrays we detected the SCL/TAL1, LMO2, Ldbl, E2A complex at all positively acting GATA-1-bound elements examined. Similarly, the SCL complex is present at all activating GATA elements in megakaryocytes and mast cells. In striking contrast, at sites where GATA-1 functions as a repressor, the SCL complex is depleted. A DNA-binding defective form of SCL maintains association with a subset of active GATA elements indicating that GATA-1 is a key determinant for SCL recruitment. Knockdown of LMO2 selectively impairs activation but not repression by GATA-1. ETO-2, an SCL-associated protein with the potential for transcription repression, is also absent from GATA-1-repressed genes but, unlike SCL, fails to accumulate at GATA-1-activated genes. Together, these studies identify the SCL complex as a critical and consistent determinant of positive GATA-1 activity in multiple GATA-1-regulated hematopoietic cell lineages. © 2009 by The American Society of Hematology.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
binding protein
LIM protein
transcription factor E2A
transcription factor GATA 1
transcription factor TAL1
animal cell
article
cell lineage
controlled study
DNA binding
erythroid cell
gene activation
gene function
gene identification
gene repression
genetic association
hematopoietic cell
knockout gene
mast cell
megakaryocyte
mouse
nonhuman
priority journal
protein depletion
repressor gene
transcription initiation
transcription regulation
Animals
Basic Helix Loop Helix Transcription Factors
Blood Cells
DNA-Binding Proteins
GATA1 Transcription Factor
Gene Expression Regulation
Immunoprecipitation
Metalloproteins
Mice
Oligonucleotide Array Sequence Analysis
Proto-Oncogene Proteins
Transcription Genetic |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein receptor |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Vakoc lab
CSHL Cancer Center Shared Resources > DNA Sequencing Service |
| Depositing User: |
CSHL Librarian
|
| Date: |
2009 |
| Date Deposited: |
19 Mar 2012 18:24 |
| Last Modified: |
30 Dec 2014 16:44 |
| PMCID: |
PMC2652367 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/25405 |
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